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polyclonal antibody against mica micb  (R&D Systems)


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    R&D Systems polyclonal antibody against mica micb
    Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either <t>polyclonal</t> anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).
    Polyclonal Antibody Against Mica Micb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+against+mica+micb/MICB+Antibody/pmc03082231-21-0-15
    Average 92 stars, based on 1 article reviews
    polyclonal antibody against mica micb - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells"

    Article Title: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/1756-9966-30-37

    Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either polyclonal anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).
    Figure Legend Snippet: Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either polyclonal anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).

    Techniques Used: Immunoprecipitation, SDS Page, Expressing

    Related Articles

    Immunoprecipitation:

    Article Title: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
    Article Snippet: The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).. Polyclonal antibody against MICA/MICB and murine monoclonal anti-MICA, anti-MICB and anti-NKG2D antibodies were purchased from R&D Systems.

    SDS Page:

    Article Title: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
    Article Snippet: The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).. Polyclonal antibody against MICA/MICB and murine monoclonal anti-MICA, anti-MICB and anti-NKG2D antibodies were purchased from R&D Systems.

    Expressing:

    Article Title: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells
    Article Snippet: The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).The cells were cultured at 37°C with 5% CO 2 in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FCS (Hyclone), 1-mM MEM sodium pyruvate solution, 2-mM MEM non-essential amino acids solution (Gibco), 0.1-mM L-glutamine, 100-U/ml penicillin and 100-μg/ml streptomycin (Gibco).. Polyclonal antibody against MICA/MICB and murine monoclonal anti-MICA, anti-MICB and anti-NKG2D antibodies were purchased from R&D Systems.



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    polyclonal antibody against mica micb  (R&D Systems)
    92
    R&D Systems polyclonal antibody against mica micb
    Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either <t>polyclonal</t> anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).
    Polyclonal Antibody Against Mica Micb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+against+mica+micb/MICB+Antibody/pmc03082231-21-0-15
    Average 92 stars, based on 1 article reviews
    polyclonal antibody against mica micb - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either polyclonal anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells

    doi: 10.1186/1756-9966-30-37

    Figure Lengend Snippet: Cervical cancer cell lines express MICA, MICB and NKG2D . CALO and INBL cells ( 1 × 10 7 ) were lysed proteins immunoprecipitated and equal amounts of protein from total lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The blots were developed with either polyclonal anti-MIC antibodies (A) or monoclonal anti-NKG2D antibodies (B) and an appropriate secondary antibody conjugated to HRP for chemiluminescence detection. Flow cytometric analysis of NKG2D expression in cervical carcinoma cell lines after 72 h induction with 10 ng MICB (C). We used only MICB to induce the expression of NKG2D because we previously obtained that MICB was a better inducer of myelomonocytic cell proliferation than MICA. Graphs show NKG2D levels (solid line) and isotype controls (dotted line).

    Article Snippet: Polyclonal antibody against MICA/MICB and murine monoclonal anti-MICA, anti-MICB and anti-NKG2D antibodies were purchased from R&D Systems.

    Techniques: Immunoprecipitation, SDS Page, Expressing